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- 作者:
李芳 ,孙玉宁 ,刘洁 ,季凯 ,朱佳荣 ,赵婷婷 ,王春莉 ,孙锦涵 - 关键词: 核呼吸因子1 血管平滑肌细胞 肺动脉 低氧 缺氧
- 摘要: 以原代小鼠肺动脉平滑肌细胞作为研究对象,采用三气培养箱模拟细胞的缺氧环境,在缺氧条件下PASMCs表型成功发生转化。Western blot结果显示,与常氧组比,缺氧组α-SMA的蛋白表达水平显著降低;缺氧条件下HIF-1α、NRF-1、mtTFA、COXIV的蛋白及mRNA的表达水平均比常氧组高。
期刊
- 作者:
Guo, Jianhui ,Yan, Yan ,Sun, Jinhan ,Ji, Kai ,Hei, Zhiping ,Zeng, Liang ,Xu, Huanzhou ,Ren, Xiang ,Sun, Yuning ( aDepartment of Biochemistry and Molecular Biology, School of Basic Medical Science, Ningxia Medical University, Yinchuan, China;bDepartment of Clinical Medicine, School of Clinical Medicine, Ningxia Medical University, Yinchuan, China;cCenter for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, Wuhan, China;dUniversity of Chinese Academy of Sciences, Beijing, China ) - 出处: Molecular Microbiology 2024 Vol.121 No.6 P1127-1147
- 关键词: Bocaparvovirus Hsc70 Hsp70 MVC virus replication
- 摘要: Minute virus of canines belongs to the genus Bocaparvovirus within the Parvoviridae family and causes serious respiratory and gastrointestinal sympt
- 作者:
黑志平 ,郭建辉 ,季凯 ,闫琰 ,王春莉 ,李芳 ,孙玉宁 ( 1宁夏医科大学基础医学院生物化学与分子生物学系;2宁夏医科大学临床医学院;3宁夏医科大学总医院呼吸及重症监护系 ) - 出处: 重庆医科大学学报 2023 第48卷 第11期 P1337-1343
- 关键词: 人鼻病毒 下呼吸道感染 儿童 临床特点
- 摘要: 目的:回顾性分析银川市下呼吸道感染患儿中人鼻病毒的流行病学及临床特征,为儿童预防人鼻病毒及临床诊疗提供理论依据。方法:采集2018至2019年因下呼吸道感染就诊于银川市妇幼保健院儿科的989例住院患儿的下呼吸道分泌物,采用多重PCR方法检测标本中病毒检出情况,分析人鼻病毒病例的基本情况、临床特征及实
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被引量:7
期刊
- 作者:
Ding, Yanqin ,Li, Na ,Sun, Jinhan ,Zhang, Linran ,Guo, Jianhui ,Hao, Xueqi ,Sun, Yuning. ( Affiliations 1 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. 2 School of Clinical Medicine, Hainan Medical University, Haikou, 571199, China. 3 Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, 750004, China. 4 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. sunraining2008@hotmail.com. ) - 出处: Virol Sin 2019 Vol.34 No.1 P78-87
- 关键词: Alcaloides/farmacologia Antivirais/farmacologia Apoptose/efeitos dos fármacos Bocavirus/efeitos dos fármacos Bocavirus/genética Quinolizinas/farmacologia Replicação Viral/efeitos dos fármacos Animais Bocavirus/fisiologia Ciclo Celular/efeitos dos fármacos Linhagem Celular Sobrevivência Celular/efeitos dos fármacos Replicação do DNA Cães Expressão Gênica
- 摘要: Oxymatrine , as the main active component of Sophoraflavescens, exhibits a variety of pharmacological properties, including anti-oxidative, anti-infla
- 作者:
高玉婧 ,李璞 ,王芳 ,吕叶 ,姚青 ,马锐 ,张曹 ,罗明秀 ,裴秀英 ,孙玉宁 - 关键词: 迁移侵袭 蛋白MIIP 肿瘤血管
- 摘要: 血管生成是肿瘤恶性转化、生长和转移的生物基础与重要环节,已成为目前抗肿瘤治疗的一个重要靶标。本项目中,我们通过一系列体内外实验手段,深入研究了迁移侵袭抑制蛋白MIIP对肿瘤血管生成的影响,并对其发挥作用的可能机制进行了探索。我们的研究结果显示,MIIP能抑制人脐静脉内皮细胞的HUVEC的迁移、侵袭和
- 作者:
马岸文 ,朱磊 ,闫琰 ,季凯 ,李芳 ,韩磊 ,孙玉宁 ( 宁夏医科大学基础医学院生物化学与分子生物学系;宁夏医科大学临床医学院;宁夏医科大学总医院呼吸及重症监护系;银川市妇幼保健院 ) - 出处: 宁夏医科大学学报 2022 第7期
- 关键词: 人副流感病毒 社区获得性肺炎 流行病学 儿童
- 摘要: 目的 分析人副流感病毒在社区获得性肺炎患儿感染中的流行病学特点及临床特征,为临床诊治和提前预防提供理论依据。方法 采集2018—2019年银川市妇幼保健院878例CAP患儿的下呼吸道分泌物,运用多重PCR的方法检测HPIV-1、HPIV-2、HPIV-3、HPIV-4等呼吸道病毒,并分析CAP患儿感
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被引量:19
- 作者:
穆乐 ,孙锦涵 ,张健 ,张淋然 ,丁彦琴 ,孙玉宁 ( 宁夏医科大学基础医学院;海南医学院临床医学院 ) - 出处: 中国动物传染病学报 2021 第29卷 第6期 P55-61
- 关键词: 犬博卡病毒 VP1结构蛋白 多克隆抗体 特异性
- 摘要: 为深入研究犬博卡病毒结构蛋白VP1及其抗体在MVC感染及致病分子机制中的作用,本研究以先期构建的MVC感染性克隆载体pI-MVC为模板,构建VP1结构蛋白原核表达载体pET32a-VP1N,转化于大肠杆菌BL21感受态细胞,IPTG诱导表达后进行蛋白可溶性分析;用镍柱亲和层析法纯化诱导表达产物,以纯
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被引量:2
期刊
- 作者:
Yanqin Ding ,Na Li ,Jinhan Sun ,Linran Zhang ,Jianhui Guo ,Xueqi Hao ,Yuning Sun ( Affiliations 1 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. 2 School of Clinical Medicine, Hainan Medical University, Haikou, 571199, China. 3 Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, 750004, China. 4 Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. sunraining2008@hotmail.com. ) - 出处: Virologica Sinica 2019 Vol.34 No.6 P729
- 摘要: In Fig. 2B, labels were misnamed in the original article. MVC label and MVC + OMT label were opposite. Now the correct Fig. 2 has been provided below.
期刊
- 作者:
Yanqin Ding ,Na Li ,Jinhan Sun ,Linran Zhang ,Jianhui Guo ,Xueqi Hao ,Yuning Sun ( Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. School of Clinical Medicine, Hainan Medical University, Haikou, 571199, China. Department of Respiratory and Critical Care, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan, 750004, China. Department of Biochemistry and Molecular Biology, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, School of Basic Medical Science, Ningxia Medical University, Yinchuan, 750004, China. sunraining2008@hotmail.com. ) - 出处: Virologica Sinica 2019
- 摘要: In Fig. 2B, labels were misnamed in the original article. MVC label and MVC + OMT label were opposite. Now the correct Fig. 2 has been provided below.
期刊
- 作者:
Fang Li ,Qian Zhang ,Qing Yao ,Long Chen ,Jianning Li ,Jianming Qiu ,Yuning Sun ( Affiliated Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan, Ningxia, China. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS 66160 USA. Electronic address: jqiu@kumc.edu. Department of Biochemistry and Molecular Biology, Ningxia Medical University, Yinchuan, Ningxia, China; Key Lab of Reproduction and Heredity of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, Ningxia, China. Electronic address: sunraining2008@hotmail.com. ) - 出处: Virus research 2014 Vol.179 P147-152
- 关键词: Bocavirus Canine minute virus DNA replication Virogenesis Infection Non-permissive cells Permissive cells
- 摘要: Canine minute virus , a kind of autonomous parvovirus, is a member of genus bocavirus in parvovirdae family. In our previous study, we constructed and
- 孙玉宁,男,汉族,1969年4月生于宁夏,(中共党员),教授,硕士生导师。2007年毕业于军事医学科学院并获哲学博士学位,历任生物化学与分子生物学系副主任、主任等职。现任基础医学院副院长,兼任中国生物化学与分子生物学学会医学分会青年委员,全国生物化学教学专业委员会理事职务。宁夏回族自治区“313人才”,生物化学与分子生物学学术带头人。成果获自治区科技进步三等奖1项,获国家专利1项。出版教材1部。发表论文30余篇,获宁夏自然科学优秀学术论文1等奖1项,二等奖1项,三等奖1项。