检索结果：
返回 34 结果。
排序：
- 作者：
Wang, Shujing ，Huang, Weidong ，Yang, Daiwen （ aDepartment of Biological Sciences, National University of Singapore, Singapore 117543, 14 Science Drive 4, Singapore;bNingxia Medical University, Yinchuan 750004 Ningxia, China ） - 出处： Journal of Biomolecular NMR 2012 Vol.54 No.4 P415-420
期刊
- 作者：
Tiantian Fan ，Ruiqi Qin ，Yan Zhang ，Jingxia Wang ，Jing-Song Fan ，Xiangli Bai ，Wensu Yuan ，Weidong Huang ，Shuo Shi ，Xun-Cheng Su ，Daiwen Yang ，Zhi Lin （ School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ; Department of Biochemistry and Molecular Biology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750004, People's Republic of China; ; State Key Laboratory of Elemento-Organic Chemistry and College of Chemistry, Nankai University, Tianjin 300071, People's Republic of China ; State Key Laboratory of Elemento-Organic Chemistry and College of Chemistry, Nankai University, Tianjin 300071, People's Republic of China ; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore; ; School of Life Sciences, Tianjin University, Tianjin 300072, People's Republic of China; ） - 出处： Proceedings of the National Academy of Sciences 2021 Vol.118 No.38 Pe2100496118
- 摘要： Natural spider silk with extraordinary mechanical properties is typically spun from more than one type of spidroin. Although the main components of va
- 作者：
海娥 ，张慧敏 ，高晓菁 ，张小敏 ，郭嘉义 ，黄卫东 （ 1宁夏医科大学基础医学院生物化学与分子生物学系；2宁夏疾病预防控制中心；3宁夏医科大学科技中心 ） - 出处： 中国病原生物学杂志 2024 第19卷 第6期 P653-658
- 关键词： 铜绿假单胞菌PAO1 PA0285蛋白 环二鸟苷单磷酸 环境信号
- 摘要： 目的 分析铜绿假单胞菌中环二鸟苷单磷酸代谢蛋白PA0285感应环境因素的作用，探讨其参与c-di-GMP信号转导的机制。方法 通过PCR技术对pa0285转座子突变菌株进行遗传背景确认；以铜绿假单胞菌野生型PAO1和2株PA0285插入失活突变菌株为研究对象，选定24种化合物，利用绿色荧光蛋白报告基
- 作者：
黄卫东 ，张文胜 ，陈一帆 ，郭嘉义 （ 1宁夏医科大学基础医学院；2苏州大学医学院；3宁夏医科大学医学科学技术研究中心 ） - 出处： 宁夏医科大学学报 2023 第45卷 第12期 P1189-1193
- 关键词： DPF2基因 CRISPR/Cas9n double nike系统 基因敲除 PANC-1细胞株
- 摘要： 目的 利用改良的CRISPR/Cas9n double nick系统构建DPF2基因敲除的PANC-1人胰腺癌细胞模型。方法 设计两对靶向DPF2基因第四外显子上下游的单链向导RNA，化学合成sgRNA寡核苷酸序列，并克隆至pGL3-U6-sgRNA-PGK-puromycin真核表达质粒中。将克隆
- 作者：
Yao, Yanxiang ，Xi, Naren ，Hai, E. ，Zhang, Xiaomin ，Guo, Jiayi ，Lin, Zhi ，Huang, Weidong （ 1School of Basic Medicine, Department of Biochemistry and Molecular Biology, Ningxia Medical University;2Research Center of Medical Science and Technology, Ningxia Medical University;3School of Life Sciences, Tianjin University ） - 出处： The Journal of general and applied microbiology 2023 Vol.68 No.5 P232-241
- 关键词： c-di-GMP PA0575 PA4601 PA4959 P aeruginosa PAO1
- 摘要： As a central signaling molecule, c-di-GMP -cyclic diguanosine monophosphate) is becoming the focus for research in bacteria physiology. Pseudomonas ae
- 作者：
席那仁 ，海娥 ，张小敏 ，郭嘉义 ，黄卫东 （ 1宁夏医科大学基础医学院生物化学与分子生物学系；2宁夏医科大学科技中心 ） - 出处： 微生物学通报 2023 第50卷 第5期 P2113-2126
- 关键词： 铜绿假单胞菌PAO1 环鸟苷二磷酸 PA2072敲除突变菌株
- 摘要： 【背景】铜绿假单胞菌为革兰氏阴性杆菌，是医院感染的常见条件致病菌之一。广泛存在于细菌中的第二信使分子环鸟苷二磷酸对细菌生理生化功能具有重要的调节作用。铜绿假单胞菌PAO1中存在参与c-di-GMP代谢的基因PA2072。【目的】探讨铜绿假单胞菌PAO1中c-di-GMP代谢相关基因PA2072的生物
-
被引量：3
- 作者：
Wang, Jingxia ，Fan, Tiantian ，Li, Xi ，Hu, Xiao Xia ，Huang, Weidong ，Yuan, Wensu ，Lin, Zhi （ 1School of Life Sciences, Tianjin University, Tianjin, 300072, China;2Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, Tianjin, 300072, China;3Analysis and Testing Center, Tianjin University, Tianjin, 300072, China;4Department of Biochemistry and Molecular Biology, School of Basic Medicine, Ningxia Medical University, Ningxia, Yinchuan, 750004, China ） - 出处： Matter 2022 Vol.5 No.12 P4396-4406
- 摘要： Silks are protein-based biopolymers with unique combinations of different physical properties. Silkworm silk is the most well studied due to its abund
期刊
- 作者：
Xiao Fan ，Wensu Yuan ，Weidong Huang ，Zhi Lin （ Affiliations 1 School of Life Sciences, Tianjin University, Tianjin, 300072, PR China. 2 Department of Biochemistry and Molecular Biology, School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China. Electronic address: weidong1969@hotmail.com. 3 School of Life Sciences, Tianjin University, Tianjin, 300072, PR China. Electronic address: linzhi@linzhi.net. ） - 出处： Biochimie 2023
- 关键词： Leptin Leptin receptor Metabolic diseases Signaling.
- 摘要： As a multi-potency cytokine, leptin not only plays a crucial role in controlling weight and energy homeostasis but also participates in the metabolic
- 作者：
Jingxia Wang ，Tiantian Fan ，Xi Li ，Xiaoxia Hu ，Weidong Huang ，Wensu Yuan ，Zhi Lin （ 1School of Life Sciences, Tianjin University, Tianjin 300072, P.R. China;2Tianjin Key Laboratory of Function and Application of Biological Macromolecular Structures, School of Life Sciences, Tianjin University, Tianjin 300072, P.R. China;3These authors contributed equally;4Analysis and Testing Center, Tianjin University, Tianjin 300072, P.R. China;5Department of Biochemistry and Molecular Biology, School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China;6Lead contact ） - 出处： Matter 2022
- 关键词： silkworm silk artificial silk fibroin silk spinning biomaterials
- 摘要： Silk-based biomaterials from silkworms are widely used in the textile and medical industries. Recently, spider silks have attracted people’s interest
- 作者：
Weidong Huang ，Yan Zhang ，Yifan Chen ，Yin Wang ，Wensu Yuan ，Ning Zhang ，Toong Jin Lam ，Zhiyuan Gong ，Daiwen Yang ，Zhi Lin （ 1 Department of Biochemistry and Molecular Biology, School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, 750004, P.R. China; 2 School of Life Sciences, Tianjin University, Tianjin, 300072, P.R. China; 3 Department of Biological Sciences, National University of, Singapore, 117543, Singapore ） - 出处： Scientific Reports 2017 Vol.7
- 摘要： A cDNA library from a pool of all the seven silk glands from a tropical spider species was constructed. More than 1000 expressed sequence tag clones
- 黄卫东， 女， 汉族， 1969年3月生于银川，教授，硕士生导师。2006年毕业于国立新加坡大学，获博士学位，2010年获宁夏回族自治区“百人计划”奖励，参与主编留学生教材一部，发表高水平论文十余篇。